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Effects of LGGp on gut barrier <t>integrity.</t> <t>Caco-2</t> cells were stimulated with 10 µg/ml LGGp for 48 (h) The TEER values were measured as follows: TEER = (measured resistance value−blank value) × single cell layer surface area (cm 2 ). The exposure to LGGp elicited a significant increase in TEER (A) . Caco-2 cells were stimulated with 10 µg/ml LGGp for 48 h (B, C, D). FITC dextran permeability was assessed in transwell plate (B) and appeared reduced after 2 h in cells pre-treated with LGGp. Cells were processed for mRNA analysis by RT-PCR. Occludin (C) and ZO-1 (D) expression levels were significantly increased in Caco-2 cells exposed to LGGp. RT-PCR analysis was performed using the comparative threshold cycle (CT) method. Gene expression was normalized against the expression of the reference gene glucoronidase beta (GUS-B). Each point represents median and error with range (A, B) or median and interquartile range (C, D) of five independent experiments. Data were analyzed using Mann Whitney U test. LGGp, heat-inactivated LGG postbiotic; TEER, Trans-epithelial electrical resistance; ZO-1, zonula occludens 1. *p<0.05 vs NT.
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Effects of LGGp on gut barrier integrity. Caco-2 cells were stimulated with 10 µg/ml LGGp for 48 (h) The TEER values were measured as follows: TEER = (measured resistance value−blank value) × single cell layer surface area (cm 2 ). The exposure to LGGp elicited a significant increase in TEER (A) . Caco-2 cells were stimulated with 10 µg/ml LGGp for 48 h (B, C, D). FITC dextran permeability was assessed in transwell plate (B) and appeared reduced after 2 h in cells pre-treated with LGGp. Cells were processed for mRNA analysis by RT-PCR. Occludin (C) and ZO-1 (D) expression levels were significantly increased in Caco-2 cells exposed to LGGp. RT-PCR analysis was performed using the comparative threshold cycle (CT) method. Gene expression was normalized against the expression of the reference gene glucoronidase beta (GUS-B). Each point represents median and error with range (A, B) or median and interquartile range (C, D) of five independent experiments. Data were analyzed using Mann Whitney U test. LGGp, heat-inactivated LGG postbiotic; TEER, Trans-epithelial electrical resistance; ZO-1, zonula occludens 1. *p<0.05 vs NT.

Journal: Frontiers in Immunology

Article Title: Postbiotic effects elicited by heat-inactivated Lacticaseibacillus rhamnosus GG against cow’s milk allergy in human cells

doi: 10.3389/fimmu.2025.1671729

Figure Lengend Snippet: Effects of LGGp on gut barrier integrity. Caco-2 cells were stimulated with 10 µg/ml LGGp for 48 (h) The TEER values were measured as follows: TEER = (measured resistance value−blank value) × single cell layer surface area (cm 2 ). The exposure to LGGp elicited a significant increase in TEER (A) . Caco-2 cells were stimulated with 10 µg/ml LGGp for 48 h (B, C, D). FITC dextran permeability was assessed in transwell plate (B) and appeared reduced after 2 h in cells pre-treated with LGGp. Cells were processed for mRNA analysis by RT-PCR. Occludin (C) and ZO-1 (D) expression levels were significantly increased in Caco-2 cells exposed to LGGp. RT-PCR analysis was performed using the comparative threshold cycle (CT) method. Gene expression was normalized against the expression of the reference gene glucoronidase beta (GUS-B). Each point represents median and error with range (A, B) or median and interquartile range (C, D) of five independent experiments. Data were analyzed using Mann Whitney U test. LGGp, heat-inactivated LGG postbiotic; TEER, Trans-epithelial electrical resistance; ZO-1, zonula occludens 1. *p<0.05 vs NT.

Article Snippet: For all experiments, we used a well validated model of gut barrier based on Caco-2 cells monolayer (American Type Culture Collection, Middlesex, UK; accession number: HTB-37) ( , ).

Techniques: Permeability, Reverse Transcription Polymerase Chain Reaction, Expressing, Gene Expression, MANN-WHITNEY

Effects of LGGp on human enterocytes differentiation. Caco-2 cells were stimulated with 10 µg/ml LGGp for 48 (h) Cells were processed for mRNA analysis by RT-PCR. Muc2 (A) and Lactase (B) expression levels were significantly increased in Caco-2 cells exposed to LGGp. RT-PCR analysis was performed using the comparative threshold cycle (CT) method. Gene expression was normalized against the expression of the reference gene glucuronidase beta (GUS-B). Each point represents median and interquartile range of five independent experiments. Data were analyzed were analyzed using Mann Whitney U test. LGGp, heat-inactivated LGG postbiotic; Muc-2, mucin 2; *p<0.05 vs NT.

Journal: Frontiers in Immunology

Article Title: Postbiotic effects elicited by heat-inactivated Lacticaseibacillus rhamnosus GG against cow’s milk allergy in human cells

doi: 10.3389/fimmu.2025.1671729

Figure Lengend Snippet: Effects of LGGp on human enterocytes differentiation. Caco-2 cells were stimulated with 10 µg/ml LGGp for 48 (h) Cells were processed for mRNA analysis by RT-PCR. Muc2 (A) and Lactase (B) expression levels were significantly increased in Caco-2 cells exposed to LGGp. RT-PCR analysis was performed using the comparative threshold cycle (CT) method. Gene expression was normalized against the expression of the reference gene glucuronidase beta (GUS-B). Each point represents median and interquartile range of five independent experiments. Data were analyzed were analyzed using Mann Whitney U test. LGGp, heat-inactivated LGG postbiotic; Muc-2, mucin 2; *p<0.05 vs NT.

Article Snippet: For all experiments, we used a well validated model of gut barrier based on Caco-2 cells monolayer (American Type Culture Collection, Middlesex, UK; accession number: HTB-37) ( , ).

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Gene Expression, MANN-WHITNEY